CONTROL-T Retreat, 22.-23.6.2018, "Landhotel-Johanneshof" Langen-Egelsbach

We are delighted to announce two guest speakers for our 2018 retreat: Jongeun Park, Hinxton/Cambridge, U.K. and Michael Floßdorf, München. Please scroll down for impressions of the meeting (photos by courtesy of M.-L. Hansmann).

Friday, June 22nd , 2018
Time Topic Speaker
from 12:00 CheckIn
12:30-13:30 Lunch Guests and CONTROL-T members
13:30-13:40 Welcome by the speaker of CONTROL-T M-L. Hansmann
13:40-14:20 RP1 Pathogenesis of angioimmunoblastic T-cell lymphoma R. Küppers
14:20-15:00 RP3 Mechanisms of T-cell homeostasis and its perturbation in lymphomagenesis S. Sagasser / J. Kirberg
15:00-15:40 RP5 The oncogenic cooperation of TCL1 with T-cell receptor signaling in T-PLL M. Herling
15:40-16:00 Break (coffee) Guests and CONTROL-T members
16:00-16:50 Human Developmental Cell Atlas: profiling thymus development at a single cell resolution J. Park
16:50-17:40 Stochastic developmental programs of T cells inferred from single-cell fate mapping in vivo M. Floßdorf
17:40-18:10 CONTROL-T cooperation talks CONTROL-T members
18:10-19:10 Dinner Guests and CONTROL-T members
from 19:10 Get together (open terrace, weather permitting) Guests and CONTROL-T members

Saturday, June 23rd , 2018
Time Topic Speaker
8:00-9:00 Breakfast Guests and CONTROL-T members
9:00-9:40 RP4 Mathematical modeling of homeostasis and oncogenesis in mature T-cells / The Bertalanffy-type Cancer Growth Model I. Roeder, M. Seifert / H.H. Diebner
9:40-10:20 The role of chemokine ligands and receptors in the dissemination of ALCL S. Hartmann
10:20-10:40 Break (coffee) Guests and CONTROL-T members
10:40-11:40 Internal discussions CONTROL-T; Congress in 2019 etc. Control-T members
12:00-13:00 Lunch
Guests and CONTROL-T members

Impressions from the CONTROL-T Retreat, 22.-23.6.2018, "Landhotel-Johanneshof" Langen-Egelsbach

Retreat2018_8Martin-Leo Hansmann (Speaker and PI RP1, right) and Sylvia Hartman (PI RP7).
M.-L. Hansmann opened and concluded the final retreat of the CONTROL-T consortium. A bit more than one year to go. Although being pleased about the progress of the research group, M.-L. Hansmann motivated all members of CONTROL-T to start the final spurt.

Retreat2018_2Members of Ralf Küppers' lab (from left): R. Küppers, Marc Weniger, and René Scholtysik.
The achievements so far of RP1 (Pathogenesis of angioimmunoblastic T-cell lymphoma) have been presented by René Scholtysik. It appears that TET2 mutations play a crucial role in the pathogenesis of AITL. AITL frequently coincides with B-cell lymphoma which indicates a common pathogenesis at the precursor level of T-cell and B-cell differentiation. NGS will cast light on this hypothesis. Although RP1 had to cope with a lack of material suitable for NGS due to the rareness of AITL, the library preparation and sequencing pipeline is now ready to be applied.

Jörg Kirberg (PI RP3, left) and Jongeun Park (Hinxton/Cambridge, U.K.) enganged in discussing J. Park's paper "Human developmental cell atlas: profiling thymus development at a single cell resolution." His principle component analysis of single cell expression data from thymic cell populations revealed that the cell subsets can be distinguished by the expression of one essential gene each. The observation of a new marker gene led to a sustained discussion.
J. Kirberg and his colleague Sven Sagasser summarized in their talks the findings regarding the "Mechanisms of T-cell homeostasis and its perturbation in lymphomagenesis" (RP3), outlined the planned TCR repertoire analysis and reported about the status of the library preparation.

In the foreground of the picture to the right, Sven Sagasser and Jongeun Park exchange their ideas on TCR RNA-sequencing.

In the background, Sylvia Hartmann (PI RP7) and Ralf Küppers (PI RP1) discussing their project progressions.

Retreat2018_4The picture to the left shows Ingo Roeder (PI RP4, middle) involved in discussions with members of Marco Herling's lab (RP5).
Sebastian Oberbeck (PhD student, RP5) gave an update of the state of the art of RP5: "The oncogenic cooperation of TCL1 with T-cell receptor signaling in T-PLL." The analyses so far reveal a pathway cooperation of TCL1A signaling and TCR-input which translate into an in vivo T-cell growth advantage. Therefore, T-PLL can arguably been conceived as (auto)antigen/(self)MHC-TCR-promoted disease with TCL1A acting as an TCR-signaling enhancer. In other words, the TCL1A-oncogene disturbes important mechanisms of homeostasis (see RP3). Based on this hypothesis, S. Oberbeck outlined the procedure of RP5 for the remainder of the CONTROL-T funding period.

Retreat2018_3Hans H. Diebner (Postdoc, RP4, left) discussing details of his presentation on "The Bertalanffy-type Cancer Growth Model" with Michael Floßdorf (TU München), our second this year's keynote speaker. In his talk "Stochastic developmental programs of T cells inferred from single-cell fate mapping in vivo," M. Floßdorf convincingly showed that the true correlations between T-cell differentiation steps can be revealed only on the basis of single-cell fate-mapping data. To the contrary, a population-based analysis leads to wrong correlations. Data collected on a single-cell level allow for an identification of the most likely differentiation paths using a mathematical approach based on probability generating functions.
H.H. Diebner advocated a "first principle" approach in search for cancer growth laws. Since goodness-of-fit criteria and predictability alone are not sufficient for a robust model validation, H. Diebner based his model derivation on fundamental metabolic considerations in line with the seminal work by Ludwig von Bertalanffy. The modified Bertalanffy-type growth model based on metabolism and allometry turns out to be congruent with more complex models and has, thus, a complexity preserving characteristic.

Retreat2018_5Members of Sylvia Hartmann's lab (from left): Sylvia Hartmann (PI RP7), Nadine Flinner, and Olga Goncharova.
O. Goncharova informed us on the current status of RP7, "The role of chemokine ligands and receptors in the dissemination of ALCL." In her presentation, based on the group's experimental observations, O. Goncharova formulated ideas about mechanisms of tumour migration with respect to ALCL which will soon be published.


CONTROL-T members and guests. (All photos on this page by courtesy of M.-L. Hansmann, Frankfurt a.M.)